Bowyer was in the top-five most of the 50-minute session but bolted to the top of the speed chart in the final minutes behind an average lap speed of 178.059 mph.
Las Vegas Motor Speedway has not been a particularly good track for Bowyer during his career. In 15 starts, he has one top-five and four top-10 finishes.
About 19 minutes into the session, the caution was displayed for debris on the track. Cars returned to action a few minutes later.
Ross Chastain was forced to sit out the final 15 minutes of practice after his No. 15 Chevrolet was late to inspection last weekend at Indianapolis.